Sapling Learning Map The isoelectric point (pl) of a migrate in an electric fiel

Question

Sapling Learning Map The isoelectric point (pl) of a migrate in an electric field. Since the peptide is zwitterionic, there are the same number of positive charges as negative charges on the peptide population. The pl can be estimated fairly accurately (within 0.1 or 0.2 pH units) from the pk values of all the proton dissociable groups in the peptide. Using pK values from the table at the right, estimate the pi value of the following hexapeptide Amino Side Acid Chain pk Arg [ 12.5 Asp 3.7 Cys 8.2 Glu His Lys Tyr Met-Asp-Lys-Arg-Ala-Tyr 4.3 6.0 10.5 10.5 peptide- 8.0 Number NH3 3.4 COOH Amino and carboxy terminal values differ from the amino and carboxy values of a single amino acid. Previous Give Up & View Solution Q, Check Answer 0 Next Exit - Hint

Explanation / Answer

The theoretical PI calculated is found to be 8.34.

just figure out at which pH the charge would become zero on the peptide and then take the closest pKa of both sides and divide it by 2.

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