Which of the following amino acids will bind to a DEAE-cellulose column (resin i

Question

Which of the following amino acids will bind to a DEAE-cellulose column (resin is positively-charged) at pH = 8.0?

Lysine, pI = 9.74

Histidine, pI = 7.6

Glutamate, pI = 3.22

Select one:

a. Lysine

b. Histidine

c. Glutamate

d. Lysine + Histidine

e. Glutamate and Histidine

In column chromatography, molecules that bound to the stationary phase must come out by:

Select one:

a. physically removing the resin away from the column

b. slowly passing many volumes of the mobile phase through the column

c. selectively modifying the properties of the mobile phase

d. increasing the flow rate of the mobile phase

Which of the following short peptides will bind more efficiently to a cation exchange column at pH=7?

Select one:

a. Ser-Glu-Tyr-Gly-Asp-Thr

b. Ser-His-Tyr-Gly-Asp-Thr

c. Ser-His-Tyr-Gly-Ala-Thr

d. Ser-His-Tyr-Gly-Lys-Thr

e. Ser-Arg-Tyr-Gly-Lys-Thr

Which of the following short peptides will bind more efficiently to a hydrophobic column?

Select one:

a. Gly-Ser-Tyr-Thr-Asn-Glu

b. Gly-Ser-Phe-Thr-Asn-Gln

c. Gly-Ser-Phe-Leu-Asn-Gln

d. Gly-Cys-Phe-Leu-Asn-Gly

e. Gly-Cys-Phe-Gly-Asn-Gly

Explanation / Answer

Answer: e. Glutamate and Histidine

Though resin used here is positively charged, so the amino acids must be negatively charged

Answer: b. slowly passing many volumes of the mobile phase through the column

Answer:

Aminoacids Total charge

a. Ser-Glu-Tyr-Gly-Asp-Thr -2

b. Ser-His-Tyr-Gly-Asp-Thr -1

c. Ser-His-Tyr-Gly-Ala-Thr 0

d. Ser-His-Tyr-Gly-Lys-Thr +1

e. Ser-Arg-Tyr-Gly-Lys-Thr +2

in cation exchange chromatography resin is negatively charge so amino acid must be positive

So answer = e. Ser-Arg-Tyr-Gly-Lys-Thr  

Answer:

d. Gly-Cys-Phe-Leu-Asn-Gly

because 3 hydrophobic amino acids are present.

Which of the following short peptides will bind more efficiently to a hydrophobic column?

Select one:

a. Gly-Ser-Tyr-Thr-Asn-Glu

b. Gly-Ser-Phe-Thr-Asn-Gln

c. Gly-Ser-Phe-Leu-Asn-Gln

d. Gly-Cys-Phe-Leu-Asn-Gly

e. Gly-Cys-Phe-Gly-Asn-Gly

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