You have an unmodified peptide that has a pI of 8.5. You then modify this peptid

Question

You have an unmodified peptide that has a pI of 8.5. You then modify this peptide such that one of the side chains in this peptide becomes phosphorylated. What would you expect the pI of this new, modified peptide to be? would it be higher, lower, or the same as the original pI of the phosphorylated peptide?

You have an unmodified peptide that has an overall charge of +3 at pH of 7.0. you then modified this peptide such that lysine side chain in this peptide becomes acetylated, and one amino acid side chain becomes carboxylated. What would you expect the overall charge of this new modified peptide to be at pH 7.

Explanation / Answer

It will be lower than the original

Acetylation means, charge becomes +3 to +2, carboxylation means it becomes +1

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