34. An antibody must be linked with an enzyme for detecting an antigen in ______

Question

34. An antibody must be linked with an enzyme for detecting an antigen in ___________

A. RIA

B. ELISA

C. agglutination           

D. Immunofluorescence assay

E. western blot imumunodetection

35. The proteomics of today are capable of _________________________.

A. identifying all the proteins that can bind a ligand if the protein can be immobilized on an array

B. identifying all the proteins expressed by a cell at a given time

C. determining the structure of all the protein translated in a cell

D. identifying all the proteins that can bind a ligand

36. How can a protein array be made?

A. Proteins chemically synthesized on the membrane

B. Through specific antibodies (immobilized on a membrane) for each of the proteins

C. Proteins spotted on the membrane like oligonucleotide probes spotted on a Southern blot

D. Tagged proteins exposed to a membrane that has immobilized ligands the tag may bind

37. To identify interacting proteins by coimmunoprecipitation, _______ proteins should be attached to the beads.   

A. one antibody against each of the

B. one antibody against one of the

38. When the protein X (i.e. any given protein) is purified from the cytoplasm of resting cells, the protein appears as a monomeric polypeptide with a relative molecular mass of 42 kD. When the protein X is isolated from mitotically active cells, the protein always gets isolated with four other polypeptide chains as evidenced by four additional protein bands in SDS-PAGE. Which of the following best explains the result?

A. Protein X is a monomer in the resting cell, a homopolymer in the dividing cells

B. Protein X is unmodified in the resting cells, variously modified in the dividing cells

C. Protein X stays/acts alone or stays/acts with multiple partners at different stages of cell growth

39. You want to make a protein array using an anti-flag antibody as the anchor (the antibody can be spotted in the membrane to capture the proteins to be screened). Which of the following steps you also must do to make the array?

A. Clone the codons for the flag (that the antibody recognizes) in-frame to each of the ORFs of the cell       

B. Clone the codons for the antibody (that recognizes the flag) in-frame to each of the ORFs of the cell

C. Make the cell extract and spot the extract on the membrane in a grid

D. Chemically attach the flag to each of the antibodies

40. In DNA gel mobility shift assays, protein and radiolabeled dsDNA are mixed and then the mixture is resolved in a native polyacrylamide gel. Which of the following indicates that the protein bound the dsDNA molecules?

A. The DNA-bound protein migrated slower than free proteins

B. The protein-bound DNA migrated slower than free DNA

C. The bound protein becomes radioactive

D. The bound DNA lost radiolabels

41. DNA gel mobility shift in the presence of excess nonspecific dsDNA dose not tell ________.

A. if the protein bound the DNA

B. where the protein bond in the DNA fragments

C. if the protein bound one or more DNA fragments                   

D. if the protein bound DNA specifically or non-specifically

42. In DNase I protection assay, DNase I cannot cut certain areas of the DNA probe because _______.

A. DNase I only cuts DNA that is bound to a protein

B. the binding protein shields certain areas of the DNA molecule

C. the DNase I enzyme got inactivated by the protein bound to DNA

43. Simple ChiP-sec technology decipher all these but not _________.

A. where the protein binds DNA relative to a gene promoter

B. if the protein binds DNA alone or with other partners

C. if a protein binds DNA inside a live cell

44. Chip-chip technology involves all the following steps except________.

A. treat of the cells with formaldehyde

B. break the chromosomes using a sonicator

C. make extract of the cell by homogenization               

D. precipitate the protein-DNA complex with an antibody

E. free the DNA fragment from protein and antibody and sequence the DNA fragments

45. Which of the technologies gives the information of the DNA sequence of the precipitated DNA fragments?

A. ChiP

B. ChiP-seq               

C. ChiP-chip

D. ChiP-seq and ChiP-chip

Explanation / Answer

34. ELISA

35. identifying all the proteins that can bind a ligand if the protein can be immobilized on an array

36.Through specific antibodies (immobilized on a membrane) for each of the proteins

37. one antibody against each of the

38. The protein X

stays/acts alone or stays/acts with multiple partners at different stages of cell growth

39. Clone the codons for the antibody (that recognizes the flag) in-frame to each of the ORFs of the cell

40. The protein-bound DNA migrated slower than free DNA

41. if the protein bound DNA specifically or non-specifically

42. the binding protein shields certain areas of the DNA molecule

43. if the protein binds DNA alone or with other partners

44. free the DNA fragment from protein and antibody and sequence the DNA fragments

45. ChiP-seq and ChiP-chip

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