when doing DNA isolation, what is the purpose to add RNase ? A. to digest RNA B.

Question

when doing DNA isolation, what is the purpose to add RNase ? A. to digest RNA B. To release RNA from cells C. To synthesize RNA The average molecular weight of DNA is 650 Dalton per base pair. With the definition of 1 Dalton=1 gram/mole, 1µg of a 1000 bp long DNA fragment has ___ molecules. Polyacrylamide gel electrophoresis can separate: A.DNA or protein B. DNA C. Protein A DNA cloning project involves a few experiments, which one below illustrates the right sequence of activitives? A. Ligation followed by transformation and then plasmid isolation B. Transformation followed by plasmid isolation and then ligation C. Plasmid isolation followed by ligation and then transformation

Explanation / Answer

1) To digest RNA - RNase is used to degrade the RNA present in the sample, so the DNA can be isolated. Likewise protease is used to breakdown proteins in the sample. Both enzymes are optionally used depending on the sample.

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