In a research lab, you will not explicitly described. Often rese always be given

Question

In a research lab, you will not explicitly described. Often rese always be given a protocol with the solutions you will need archers have to adapt to the information that is provided in a been given the following information: oata solution. For the following questions, assume you have 4X TGB (Tris-Glycine Buffer) pH 8.3 Tris Glycine SDS 25mM 250mM 0.1% How many milligrams of SDS will you add when making 500mL of 4X SDS is 288.38)? (2pts) 3) TGB? (FW of 4) If you were to make a 1X working solution of TGB, at what concentration will each component be? (2pts) Tris-base = Glycine = SDS =

Explanation / Answer

3) to make 0.1 percent (w/v) 500ml sds stock solutio(4x) =2gm or 2000mg of sds is required.

4) If we make 1× working solution of sds buffer the concentration of components given above will be

Sds 0.1 percent

Tris base 25 mM

Glycin 250 mM

The concentrations are like this because we have 4× stock i.e actual conc. of Tris base is 100 mM in 4× stock not 25 mM beacausewe are making stock 4 time more concentrated .Due to this when we make 1× working buffer from 4× by diluting it 4 times the final concentaconce of Tris base will become 25 mM .Same logic applied on sds and glycin concentration as well.

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