6. MCQ. Choose the right answer: Which peptide has the greatest absorbance at 28

Question

6. MCQ. Choose the right answer: Which peptide has the greatest absorbance at 280nm? a) Gln-Leu-Glu-Phe-Thr-Leu-Asp-Gly-Tyr b) Ser-Val-Trp-Asp-Phe-Gly-Tyr-Trp-Ala c) His-Ala-Tyr-Phe-Ala-Ser-Cys-Trp-Gly d) Ala-Trp-Lys-Tyr-Gln-Asn-Trp-Trp-Arg The units of the molar extinction coefficient are usually expressed in: a) M. cm1 b) M. cmm A. B. c) M1. cm1 d) M1. cm what would be the absorbance of 0.1mg/ml solution of BSA (E%-6.7%cm-1)? C. a) 0.067 b 0.67 c) 6.7 d) 67 D. Which of the followings interferes Bradford (Coomassie dye binding) protein assay? d) RNA a) Dithiothreitol b)B-mercatoethanol c)SDS Absorbance of proteins at 280nm is mainly due to the presence of a. tryptophan b. tyrosine c. cystine d. all of above b)

Explanation / Answer

A.)b.)because it contains more Trp and aromatic residues.

B.)c.)M1cm1

D.)c.)SDS

E.)d.)all of above

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