You pick one o following nutrient pools. (Each row and column is a separate pool

Question

You pick one o following nutrient pools. (Each row and column is a separate pool. So for e alanine, tryptophan and phenylalanine, while pool 4 contains alanine, guanine and threonine.) 4. f your auxotrophic mutants and test it for growth on minimal media supplemented with the xample, pool I Pools 4 5- threonine leucine histidine alanine tryptophan valine line alanine The mutant colony grows on media supplemented with either nutrient pool 2 or 5. What single nutrient does this demonstrate that the mutant is auxotropic for? Explain your reasoning. (2 pts) Poul 2:uanine,voline, proling RotS:trytophan, Vuline, teinc

Explanation / Answer

Auxotrophic Mutants are tested for growth on minimal media which have been supplemented with the given nutrient pools. Since each row and column is a separate pool we can deduce that the combination of these pools can be used to figure out the common nutrient that the mutant requires if it wants to grow. The intersection of any one row and column offers a unique nutrient that any organism growing on the corresponding nutrient pools will be auxotrophic to.When the mutant colony grows on media supplemented with either Nutrient Pool 2 or Nutrient Pool 5 we can see that the Nutrient Pool 2 contains Guanine, Valine and Proline, while Nutrient Pool 5 contains tryptophan, valine and leucine. Now valine is the single nutrient that the mutant colony requires and is thus auxotrophic for this particular setup. This setup is also very characteristic of the ANOVA system wherein every variable has a unique pattern to it which helps in discerning a change across two or more variables.

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