You pick one of your auxotrophic mutants and test it for what nutrient it needs

Question

You pick one of your auxotrophic mutants and test it for what nutrient it needs to grow. So that you don't have to do 20 different plates, you use a pooled nutrient strategy. Yeast are put on minimal media supplemented with the following nutrient pools. (Each row and column is a separate pool. So e.g., pool 1 contains alanine, tryptophan and phenylalanine, while pool 4 contains alanine, guanine and threonine) This mutant colony grows on media supplemented with either nutrient pool 2 or 5, what single nutrient does this demonstrate that the mutant is auxotropic for? Explain your reasoning,

Explanation / Answer

Yeast strain is auxotrophic for VALINE.

The pool is made up of glutamate, VALINE and proline and pool 5 is made up of tryptophan, VALINE and leucine. And these are the only pools that the strain could grow. And the common amino acid is VALINE. So it can be concluded that VALINE is the component that the strain is auxotrophic for.

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