5. _____A titration curve for the amino acid cysteine will reveal a total of thr

Question

5. _____A titration curve for the amino acid cysteine will reveal a total of three (3) pKa’s.

6. _____ Adding urea to a solution containing the protein albumin, is likely to stabilize the three-dimensional structure of the protein.

7. _____ The distal histidine in a subunit of hemoglobin can form a hydrogen bond with the dioxygen (O2) bound to the heme, thus stabilizing oxyhemoglobin (HbO2).

8. _____ Water has a lower heat of vaporization than compounds of similar molecular weight because of water’s tendency to form hydrogen bonds.

9. _____ The oxygen-dissociation curve for hemoglobin has a sigmoidal shape because oxygen binding is altered by the content of CO2.

10. _____ Comparing kcat/KM ratios with different substrates can help determine substrate “preference” for a given enzyme.

Explanation / Answer

5. True
Cysteine has three ionizable groups.

6. False
Urea denatures tertiary structure of proteins.

7. True
Distal histidine stabilizes bound oxygen molecule in Hemoglobin.

8. False.
Water has high heat of vaporization = 40.65 kJ/mol

9. False.
The sigmoidal behavior of oxygen binding is due to cooperative binding.

10. True
Kcat/Km is used to compare the preference of an enzyme for related substrates.

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