This is a practice question for a cell biology course that I was hoping I could

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This is a practice question for a cell biology course that I was hoping I could get help with.

PRACTICE QUESTION 5 You have isolated four membrane proteins (A, B, C, and D) that you suspect are involved in the transport of Mg and/or Ca across membranes. You produce four types of liposomes that contain protein A. protein B, protein C, or protein D as the only membrane protein. For all of the following experiments each liposome is prepared with an internal solution of 100 mM Mg and 100 mM Ca2 A. You incubate liposome A (containing protein A) in a solution of 100 mM Mg and 100 mM Ca2 outside of the membrane. After 1 hr you measure the internal ion concentrations and find 100 mM g and 100 mM Ca. You repeat the experiment but this time with additional 100 mM ATP in both the internal and external solutions. After 1 hr the internal concentrations are 125 mM Mg and 75 mM Ca. Explain how protein A functions in membrane transport of ions. B. You incubate liposome B (containing protein B) in a solution of 100 mM Mg and 100 mM Ca-outside of the membrane. After 1 hr you measure the internal ion concentrations and find 100 mM Mg and 100 mM Ca2-. You repeat the experiment but this time with additional 100 mM ATP in both the internal and external solutions. After 1 hr the internal concentrations are 50 mM Mg and 125 mM Ca2. Explain how protein B functions in membrane transport of ions C. You incubate liposome C (containing protein C) in a solution of 200 mM Mg and 200 mM Ca2 outside of the membrane. After 1 hr you measure the internal ion concentrations and find 100 mM Mg and 150 mM Ca2. You repeat the experiment but this time with additional 100 mM ATP in both the internal and external solutions. After 1 hr the internal concentrations are 100 mM g and 150 mM Ca. Explain how protein C functions in membrane transport of ons D. You incubate liposome D (containing protein D) in a solution of 200 mM Mg2 and 200 mM Caoutside of the membrane. After 1 hr you measure the internal ion concentrations and find 150 mM Mg2- and 50 mM Ca*, You repeat the experiment but this time with additional 100 mM ATP in both the internal and external solutions. After 1 hr the internal concentrations are 150 mM Mg2' and 50 mM Ca2. Explain how protein D functions in membrane transport of ions.

Explanation / Answer

1. CaMg ATPase will be the enzyme

2. MgCa ATPase will be the enzyme

3. CaMg symporter will be the enzyme

4. Mg Ca symporter

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