7. Complete the following conversions: L -0.003 m c, 250 ml 8. Put the following
ID: 195968 • Letter: 7
Question
7. Complete the following conversions: L -0.003 m c, 250 ml 8. Put the following volumes in order from smallest to largest. 2.5 mL; 250 0.025 mL; 2.5 a. b. 100 ul, 0.01 mL; 250 0.015 mL 9. Explain the reason for each of the following rules: a. Always set the micropipette within its designated range. b. Always use a micropipette with a tip. c. Always hold a loaded micropipette in a vertical position. d. Always release the micropipette plunger slowly. 10. Why is it important to balance a microcentrifuge before turning it on? 11. Show how you would arrange the indicated number of tubes in each centrifuge rotor to balance the load. If you decide that you must add tubes, explain and/or draw added tubes in. a 3 tubes b.4 tubesc.5 tubes 4. 10 e. 5 tubesExplanation / Answer
7. a) 1 uL = 0.001 mL b) 100 uL = 0.1 mL c) 250 uL = 0.25 mL
d) 1500 uL = 1.5 mL e) 60 uL = 0.06 mL f) 3 uL = 0.003 mL
8. a) 2.5 uL < 0.025 mL < 250 uL < 2.5 mL
b) 0.01 mL < 0.015 mL < 100 uL < 250 uL
9. a) Setting the micropipette outside the designated range will damage the torsion based spring mechanism inside the micropipette.
b) because a sample cannot be properly collected or deposited without a tip to hold it.
c) you cannot correctly measure the load in a horizontal position. This will introduce error in your experiment.
d) plunger is released slowly to avoid the formation of air bubbles.
10. The centrifuge is balance because imbalance at low speed can cause wobbling and a loud noise, while at high speeds, it can cause failure of the centrifuge with serious impacts on safety. In both cases, irreparable damage can be caused to the centrifuge.
11. a) a tube in each diagonally opposite end with one blank-load.
b) one tube each in diagonally opposite holes arranged in a "+" or "×" shape.
c) every hole is filled with a blank load in one hole.
d) a tube each in diagonally opposite holes.
e) a tube each in diagonally opposite holes with one blank load.
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