Eukaryotic Elongation of mRNA Transcript o Is the chemical reaction for polymeri

Question

Eukaryotic Elongation of mRNA Transcript

o Is the chemical reaction for polymerization of RNA in eukaryotes different from that in prokaryotes? How?

o How is the speed of transcription regulated?

• Prokaryotic Termination of Transcription

o Describe the process of -dependent and -independent termination of transcription. Be specific regarding the necessary characteristics of pause-sites, rut sites, and the DNA•RNA complex for each process.

• Eukaryotic Termination of Transcription

o How is termination of a gene transcript terminated by CPSF protein?

What is the role of the consensus sequence in mRNA?

Does transcription of RNA necessarily stop after the action of CPSF protein?

Explanation / Answer

Polymerization of RNA

Difference between Eukaryotic and Prokaryotic RNA polymerase:

Prokaryotic RNA polymerase:

Eukaryotic RNA polymerase:

How is the speed of transcription regulated?

Rate of transcription is controlled by many transcription factors which helps RNA polymerase to initiate the synthesis or prevents the synthesis. Thus these transcription factors play a major role in the regulation of transcription.

p-dependent and p-independent termination:

p-dependent (rhofactor dependent) termination:

p-independent (Rho independent) termination:

CPSF protein:

CPSF protein - cleavage and polyadenylation specificity factor and cleavage stimulation factor with the other termination factors cleave the mRNA from the transcription complex.

Though the mRNA is cleaved from the transcription complex, it continues to transcribe by adding poly A residues, known as poly adenylation.

Role of consensus sequence in mRNA:

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