uestion 21 Dr Vlad is testing the effects of two drugs named D1 and D2 on a prot

Question

uestion 21 Dr Vlad is testing the effects of two drugs named D1 and D2 on a protein P found in the blood. He does several tests to characterize how the drugs interact with protein P. He tests them determined that one protein P molecule has only one only with one molecule of D2 binding site for D1. Similarly, one protein P interacts a) (2pts) First, Dr Viad, tests the interaction of D1 with P in his lab. At equilibrium: the concentration of free D1 is 10 mM, and the concentration of free P is 50 mlM Given that the affinity constant is for this interaction is Kd-20 mM under these experimental conditions (pH salt, temperature, etc), what is the concentration of complex PD1 at equilibrium? x 25 The concextaction of compx aibrm would be 25M b) (2pts) Then Dr Vlad adds more D1 drug is added to the mixture. When the equilibrium is established again, the concentration of free D1 is now 20 mM, What percentage of P remains free at this new equilibrium? Explain. Note: you need to know that the experimental conditions (pH salt, temperature, etc) in part b are the same as in part a. 'The percutage of pre nains ree at me new eqlibuM 1 35% [201×2,2 20JL×20 P-357

Explanation / Answer

ANS B)    33%    ( Total => 50 + 25 = 75, Now 25 is 33% of 75)

ANS C)    37.5 mM     (half of 75)

ANS D)        Kd value for PD1 = 20mM,

             but Kd value for PD2 = 1*10-12 mMwhich is very very low than Kd value of PD1

             which means that D2 has highest affinity for P as of low Kd value.

ANS E)      

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