What is the role of IF1 and IF3 in initiation of protein synthesis in bacteria?

ID: 197946 • Letter: W

Question

What is the role of IF1 and IF3 in initiation of protein synthesis in bacteria? Pick ALL that apply

a. They bind to the 30S subunit of the ribosome and directly promote the 50S subunit to bind

b. they bind to the 30S subunit of the ribosome and prevent the 50S subunit from binding

c. they bind to the 30S subunit of the ribosome and prevent IF2-GTP from binding to the 30S subunit

Why is IF2-GTP required to initiate translation in bacteria?

a. it helps catalyze peptide bond formation between fMet and the next amino acid in the polypeptide chain

b. it brings fMet-tRNAf to the partial P site on the 30S subunit

c. It prevents mRNA from binding to the 30S subunit

d. it brings fMet-tRNAf to the partial A site within the 50S ribosome

What is the role of elongation factor tu (EF-Tu) in prokaryotic cells?

a. To guide the appropriate aminoacyl-tRNA to the A-site of the ribosome

b. To create the peptide bond between two amino acids in the A and P sites of the ribosome

c. To verify the identity of the amino acid bound to an aminoacyl-tRNA

d. To facilitate the leaving of an amino acid from the exit site

ANSWERS->

1.(b) i.e IF1 and IF3 bind to the 30s and prevents the binding of 50s

2.(b) i.e it helps in bringing fMet-tRNAf to the partial p site of the 30s subunit

3.(a) i.e EF-Tu catalyzes the binding of aminoacyl tRNA to the a site of the ribosome.

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