Before the integration of a transposable element into host DNA, its transposase

Question

Before the integration of a transposable element into host DNA, its transposase makes a staggered cut at the target site. If the staggered cut is at the sites of the arrows below, draw what the sequence of the host DNA will be after the element has inserted and subsequently excised.

13.2 Before the integration of a transposable element into host DNA, its transposase makes a staggered cut at the target site. If the staggered cut is at the sites of the arrows below, draw what the sequence of the host DNA will be after the element has inserted and subsequently excised. AATTTGGCCTAGTACTAATTGGTTGG TTAAACCGGATCATGATTAACCAACC

Explanation / Answer

Sequence is:

AATTTGGCCTAGTACTAATTGGTTGG

TTAAACCGGATCATGATTAACCAACC

The cut is made between C and T on one strand and T and A on the other.

AATTTGGCC        TAGTACTAATTGGTTGG

TTAAACCGGATCATGATT        AACCAACC        

Suppose, the transposable element is

TTTTCCCC

AAAAGGGG     

The transposable element is inserted in the host DNA  

AATTTGGCC                      TTTTCCCCTAGTACTAATTGGTTGG

TTAAACCGGATCATGATTAAAAGGGG                  AACCAACC  

The gaps are filled by host DNA polymerase

AATTTGGCCTAGTACTAATTTTCCCCTAGTACTAATTGGTTGG

TTAAACCGGATCATGATTAAAAGGGGATCATGATTAACCAACC  

After excision of the transposable element, the host DNA looks like

AATTTGGCCTAGTACTAATAGTACTAATTGGTTGG

TTAAACCGGATCATGATTATCATGATTAACCAACC  

Hence, Final sequence of the host DNA after insertion and excision is :

AATTTGGCCTAGTACTAATAGTACTAATTGGTTGG

TTAAACCGGATCATGATTATCATGATTAACCAACC  

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