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The data for the trihybrid cross below is given. By convention, only the alleles

ID: 198977 • Letter: T

Question

The data for the trihybrid cross below is given. By convention, only the alleles contributed by the heterozygous female parent is given since that will determine the phenotype of the progeny Use the data to calculate and draw a genetic map for the three gene loci, yellow body (), white eyes (wh), and cut wings (c) The genes occur in the order given and all of the mutations are recessive. Trihybrid Test Cross: Progeny Phenotypes r calculations below 803 y wht 795 16 185 179 Total 2000 Draw a genetic map for the loci y, wh, and ct based on your calculations of recombination frequencies between y-wh and wh-ct. Show your work above and draw the map below for full points. Calculate the interference below

Explanation / Answer

Gene order - y wh ct

single cross over between y and wh = 18 + 16= 34

single cross over between wh and ct = 185 + 179 = 364

Double cross over = 3 + 1 = 4

recombination frequency = (Single cross over + Double cross over/ total offspring)*100

recombination frequency between y and wh = (34+4/2000)*100 = 1.9cM

recombination frequency between wh and ct = (368/2000)*100 = 18.4cM

Interference = 1 - (observed DCO/expected DCO)

Interference = 1 - (3+1)/ [(0.019) (0.18) (2000)]

Interference = 1- 0.58 = 0.42

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