The data below were collected for an enzyme-catalyzed reaction. This graph is pr

Question

The data below were collected for an enzyme-catalyzed reaction. This graph is provided for your use anly. It will not be graded. fit is not necessary to use a graph to answer.) 0.25 2.00 0.503.33 1.00 CHck on the activate the Tools menu. 5.00 6.67 0.6 Choose "Point" and then click a place on the graph to place the point. . To move a point choose "Select," and then drag the point. Use "Select" to scroll over a point to see its coordinates or to see the equation of a line Use the option "Line" to draw a line. 2.00 Determine Vmax and Km for this reaction. 04 Number mM.s 0.2 Number 0.0 mM

Explanation / Answer

Lineweaver- burk plot is created for determining the KM and Vmax

1/V= (KM/Vmax)*1/S + 1/Vmax, Vmax= max velocity S= substrate concentration

so a plot 1/V vs 1/S gives straight line whose slope is Km/Vmax and intercept is 1/Vmax. the plot along with the data points are shown below.

from the equatino of best fit, 1/Vmax ( intercept) =0.1, Vmax= 1/0.1= 10 mM/sec, slope is KM/Vmax= 0.1, KM= 0.1*10 =1 mM

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