Questions: 1. For the microbiota project, the PCR reaction that amplifies 165 rR

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Questions: 1. For the microbiota project, the PCR reaction that amplifies 165 rRNA gene would result in: A. A mixture of various 165 rRNA genes from several strains of bacteria. Asingle 16S rRNA gene from one bacterial strain. B. For questions 2-3: Zach wants to study the microbiome inhabiting Pensacola Beach. He isolates DNA from the beach sand. He then determined the soil DNA concentration as 250 ng/ul. To set up PCR reaction for amplifying 16S rRNA genes, he needs to prepare a few reagents and formulate the reaction. 2. (Bonus point question) To prepare DNA template with the concentration of 10 ng/ul: Zach should add-1 of the original soil DNA (conc. : 250 ng/) and of water into a tube to have final volume of 100 1 ofthe soil DNA that has the concentration of 10 ng/ul. 3, Zach then uses 10 1 of DNA prepared from question 2 to set up his PCR reaction with the following formula PCR reaction to 165 rRNA gene Reagent DNA (10 ngul Primer 2 (20 pmole/u) Primer 1492R (20 pmole/u) 10X PCR buffer (dNTPs i reaction 10 Taq Polymerase (1 unit/ Total Volume (in 1X PCR 0.3 50 To use the formula above, Zach should add ul of 10X PCR buffer, and -sal of water 4. It took about 3 hours to finish PCR; Zach then wanted to use gel electrophoresis to check his 1.5 kb long PCR product. To prepare a 0.8 % agarose gel, what would be the amount of agarose that he would need for preparing the S0 mL gel solution? A. 8g 8. 4g C. 0.8 g D. 0.4g

Explanation / Answer

9) the correct option is option (b) the resistance will come from the ampicillin..

8) the correct option is option (c)

7) the correct option is option (c)

5) the correct option is option (b)

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