Biochemistry: Finding the dye concentration (µg/mL). Three dilutions were made u
ID: 199493 • Letter: B
Question
Biochemistry: Finding the dye concentration (µg/mL).Three dilutions were made using a stock solution of 150 µg/mL and water. For the first dilution, 2 µL of stock was diluted with 3 mL of water. For the second dilution, 5 µL of stock was diluted with 3 mL of water. For the third dilution,10 µL of stock was diluted with 3 mL of water.
Find the dye contration after each dilution. Answer in µg/mL. Please show work, I do not know how to approach the problem. Biochemistry: Finding the dye concentration (µg/mL).
Three dilutions were made using a stock solution of 150 µg/mL and water. For the first dilution, 2 µL of stock was diluted with 3 mL of water. For the second dilution, 5 µL of stock was diluted with 3 mL of water. For the third dilution,10 µL of stock was diluted with 3 mL of water.
Find the dye contration after each dilution. Answer in µg/mL. Please show work, I do not know how to approach the problem.
Three dilutions were made using a stock solution of 150 µg/mL and water. For the first dilution, 2 µL of stock was diluted with 3 mL of water. For the second dilution, 5 µL of stock was diluted with 3 mL of water. For the third dilution,10 µL of stock was diluted with 3 mL of water.
Find the dye contration after each dilution. Answer in µg/mL. Please show work, I do not know how to approach the problem. Three dilutions were made using a stock solution of 150 µg/mL and water. For the first dilution, 2 µL of stock was diluted with 3 mL of water. For the second dilution, 5 µL of stock was diluted with 3 mL of water. For the third dilution,10 µL of stock was diluted with 3 mL of water.
Find the dye contration after each dilution. Answer in µg/mL. Please show work, I do not know how to approach the problem.
Explanation / Answer
Answer
1. For the first dilution, 2 µL of stock was diluted with 3 mL of water.
In this 2 µL added in 2998 µL Water
2/3000 = 1/1500 = 1:1500 times dilution.
2. For the second dilution, 5 µL of stock was diluted with 3 mL of water.
In this 5 µL added in 2995 µL Water
5/3000 = 1/600 = 1:600 times dilution.
3. For the third dilution,10 µL of stock was diluted with 3 mL of water.
In this 10 µL added in 2990 µL Water
10/3000 = 1/300 = 1:300 times dilution.
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