Biochemistry: Ploypeptide sequence vDraw the sequence of the native polypeptide,

Question

   Biochemistry: Ploypeptide sequence

vDraw the sequence of the native polypeptide, indicating which positions might not be determined by these data.

vA decapeptide has the following amino acid composition:

( Arg, Gly2, His3, Leu, Met, Phe2)

§Reacting the native peptide with FDNB and then hydrolyzing released 2,4-dinitrophenylglycine.

§Incubation with CNBr yielded two fragments, a tetrapeptide with composition (Gly2, Met, Phe) and a hexapeptide.

•The tetrapeptide yielded 2,4-dinitrophenylglycine

•The hexapeptide yielded 2,4-dinitrophenylleucine

§Proteolitic cleavage by trypsin of the native peptide gave two fragments, a hexapeptide and a tetrapeptide

•The tetrapeptide yielded 2,4-dinitrophenylphenylalanine

§Pepsin cleavage of the native peptide gave a dipeptide composed only of glycine and two tetrapeptides.

N _ _ _ _ _ _ _ _ _ _ C

Explanation / Answer

The sequence if the peptide is Gly-Gly-Met-Phe-Leu-Arg-Phe-His-His-His. This is in the order of N to C terminus. Each of the reagents used above have specific properties. Carefully reading the above data will reveal the sequence of the protein as mentioned above.

"A malaria biologist"

Related Questions

Navigate

• Browse (All)
• Browse B
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.