This is the correct lab report? I do n\'t know how can I use the CSE style citat

Question

This is the correct lab report? I do n't know how can I use the CSE style citations for my lab report?

the lab report of Gram Stain in Microbiology in past tense in the passive voice without any personal pronouns( "I")

Gram staining was done on February 15th involved four steps. Before started the procedure, was washed the hands, disinfected the table with disinfectant provided at the table, and was worn the appropriate personal protective equipment (PPE). Two slides were left. One for sterile water; put a little sterile water on it, the second for smear that was written the number of bacteria tube, (#11, Pseudomonas aeruinosa), the type of medium which was a slant, and drowned a circle with a marker. Then turned on the sun-burner with the best proper flame, small blue cone. Hold the loop on the sun-

report burner for sterilization and waited for metal glow orange-red. It was kept it for 30 second to get cold because the heat kills the microbe. When the loop got cold, put it inside of the slant tube to take the culture and mixed and spread it with water and was allowed the smear to air dry. When the smear was air-dried, hold the slide at one end and pass the entire slide through the flame of a Bunsen burner two to three times with the smear-side up. Then the smear was flooded with primary stain; Crystal violet that the first dye was applied in deferential staining all cells and was waited for 1 minute- rinsed with water to remove excess dye. Then the smear was flooded with a solution called Gram's iodine and was waited for a minute - rinsed. Then a decolorizing agent—ethanol was briefly added to remove the dye-iodine complex from Gram-negative, but not Gram-positive- rinsed after 5 seconds. Then red dye safranin; the counter-stain was used and waited for a minute - rinsed. Completely, dried with bibulous paper and was viewed the smear under a light microscope. Used oil immersion at 1000x to see the cell morphology.

Result

Gram staining is an important tool in the process of bacterial identification by diving bacteria their morphological types (coccid or rod-shaped) to be clearly seen. Through a number of observations, Gram observed that certain bacteria, Pseudomonas aeruinosa was Basillus or rod which looked diplobacilli with pink color which was approved the stain is negative. ­­

Conclusion

To understand how the Gram stain reaction affects Gram-positive and Gram-negative bacteria based on the biochemical and structural differences of their cell walls. It was needed to avoid some errors such as incorrect time period for decolonization or incomplete rinsing which caused incorrect result in practical lab exam on February 15th. Unfortunately, incompletely rinsing was caused when was looked at the slide under the microscope for the second slide, the bacteria, Pseudomonas aeruinosa appeared diplococci with purple color, and it was caused changing the result incorrectly.

References

Gram’s Stain: History and Explanation of the Fundamental Technique of Determinative Bacteriology (PDF Download Available). Available from: https://www.researchgate.net/publication/270105561_Gram's_Stain_History_and_Explanation_of_the_Fundamental_Technique_of_Determinative_Bacteriology [accessed Feb 18 2018].

Pelczar J. Michael, Chan E.C.S, Krieg R. Noel, Microbiology, fifth Edition, Tata McGraw-Hill Publishing Company Limited

Ananthanarayanan R, Text book of microbiology, sixth edition, Longman Private Limited

http://www.microbelibrary.org/microbelibrary/files/ccImages/Articleimages/shoeb/Gram.html

Microbiology: A Human Perspective 7th edition by, Eugene Nester (pp47-49)

Explanation / Answer

Gram staining done on February 15th, involves four steps. Before the start of procedure, hands were washed, table was disinfected with disinfectant (provided at the table), and appropriate personal protective equipment (PPE) was worn. Two slides were provided; one for sterile water, and the second for the smear that was provided for bacterial tube, (#11, Pseudomonas aeruinosa).
The sun-burner with the best proper flame was turned on. The loop was held on the sun-burner for sterilization and wait for metal to glow orange-red. It was kept for 30 seconds to sterile it. When the loop got cold, it was put inside the slant tube to take the culture and was mixed and spread with water. The smear was allowed to air dry. When the smear was air-dried, the slide was held at one end and the entire slide was passed through the flame of a Bunsen burner two to three times, with the smear-side up. The smear was then flooded with primary stain; Crystal violet . After 1 minute the slide was rinsed with water to remove excess dye.The smear was then flooded with a solution called Gram's iodine and after one minute it was rinsed.
A decolorizing agent—ethanol was briefly added to remove the dye-iodine complex.It was rinsed after 5 seconds. The counter-stain( safranin) to stain gram negative cells was used and after one minute it was rinsed. The slide was dried with bibulous paper and was viewed under a light microscope in oil immersion at 1000x ,to see the cell morphology.

Result

Gram staining is an important tool in the process of bacterial identification. Through a number of observations,it was observed that certain bacteria like Pseudomonas aeruinosa was bacillus or rod which looked diplobacilli with pink color, which approved that the bacteria is gram negative.

Conclusion

How the Gram stain reaction affects Gram-positive and Gram-negative bacteria is based on the biochemical and structural differences of their cell wall.
Some errors such as incorrect time period for decolonization or incomplete rinsing which caused incorrect result should have been avoided in practical lab exam on February 15th. Due to incomplete rinsing, the second slide under the microscope, the bacteria Pseudomonas aeruinosa appeared diplococci with purple color, and it resulted in incorrect identification of bacteria.

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