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23 Question(2 points) mical principle that protein structure determines protein

ID: 199696 • Letter: 2

Question

23 Question(2 points) mical principle that protein structure determines protein function is illustrated by green fluorescent protein (GFP), which is an autofluorescent protein isolated from the Pacific Northwest jellyfish Aequorea victoria. GFP is a B-barrel protein containing in its interior its own chromophore (part of a molecule responsible for producing color). The chromophore forms spontaneously through a chemical reaction involving three amino acid residues. Expression of the cloned GFP gene in most cells results in the emission of green light (-510 nm) after excitation with blue light (-470 nm). In other words, as long as the protein maintains its structure, it performs the same function, regardless of the organism from which it originates.

Explanation / Answer

Both the multiple choice has C as correct answer because GFP can have green colour fluorescence in white light and UV light. As a result of potential to form an internal chromophore.

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