M/ boss has given me the purified extracellular portion of the Notch receptor I
ID: 200147 • Letter: M
Question
M/ boss has given me the purified extracellular portion of the Notch receptor I have been told I need to purify Delta to keep my job. I have a problem Delt Notch oind but do not catalyze a chemical reaction with easy to identify products. The only way to know if they work is to know if they bind together. I am s So not a problemi Following purification, I would likely do which of the following to assay if Delta binds to Notch? Pick ALL that apply I would look to see if the individual proteins have changed size on a denaturing gel I would see if ADP were produced using an assay for luciferase activity (ight from the firefly protein) 1 would use FRET and label Delta with the donor chromophore (light producing) and Notch with the acceptor molecule (light quenching) I would look for the generation of miore light would look for the quenching oflight (less light). lick Save and Submit to save and submit. Click Save All Answers to save all answers.Explanation / Answer
Hi
The options are 3 and 5.
The FRET analysis will tel whether the proteins bind to each other. In that we look for reduction in light due to quenching.
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.