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What is meant by the term heteroduplex DNA? Check all that apply. A) Heteroduple

ID: 200528 • Letter: W

Question

What is meant by the term heteroduplex DNA?

Check all that apply.

A) Heteroduplex DNA is a single-stranded DNA. B) Heteroduplex DNA is usually considered to be generated by DNA replication. C) Heteroduplex DNA is a double-stranded DNA duplex that usually contains small sequence variations or mismatched base pairs. D) Heteroduplex DNA is usually considered to be generated by pairing or annealing of two homologous DNA strands. E) Heteroduplex DNA is a double-stranded DNA duplex that usually does not contain sequence variations or mismatched base pairs.

Explanation / Answer

The correct answer is C option.

Heteroduplex DNA is a double stranded DNA duplex that usually contains small sequence variations or mismatched base pairs.

Heteroduplex DNA can be obtained from genetic recombination of single complementary strands derived from different homologous chromosomes or the chromosomes from different organisms.

It is during the process of strand invasion that regions of new duplex DNA are generated. This DNA often contains some mismatched base pairs and it is often called as Heteroduplex DNA.

All the remaining options are false with regard to heteroduplex DNA. It can never be single stranded as the name itself implies and it is generated during the time of recombination and not during the process of replication. And it often contains mismatched base pairs. It is generated from two different homologous chromosomes.

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