Help please!! I am really stuck on this dilution problem for microbiology. I kno
ID: 200907 • Letter: H
Question
Help please!! I am really stuck on this dilution problem for microbiology. I know what I have to use the equation C1V1=C2V2, but I am having trouble determining what varibale fits where. (I know that C1= 599600 and C2= 1000, but I can't figure out the volume variables) Thank you in advance!
Here is the question:
You have 5996000 CFU/ml in an overnight culture. You need to inoculate 100 microliters of sterile LB to achieve 1000 CFU/ml. What volume of overnight culture will you need to us in your inoculation? Furthermore, How would you accurately deliver the correct number of cells using a p1000, p200, or p20 within its pipetting limits?
Explanation / Answer
in your question you have given two different values for C1. I'm assuming the first value, i.e. 599600 is the correct value.
C1V1 = C2V2 [we have to find V1. and V2 = 100uL = 10-1mL]
599600 * V1 = 1000 * 10-1
V1 = 0.00016mL = 0.16uL
To accurately deliver such a small volume of overnight culture with a pipette within it's limit, we'll have to dilute the culture with distilled water.
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