What sites in the multi-cloning site could you use to cut Rpp29 out of the plasm

Question

What sites in the multi-cloning site could you use to cut Rpp29 out of the plasmid at its 3’ end (assume none of the sites are present in the Rpp29 sequence)?

PEGFP-C1 Vector Information PT3028-5 GenBank Accession #: U55763 Catalog #6084-1 Ase l SnaBI (341)Nhe 1 (592) ApaLI Eco47 II (597) Age l (601) CMV IE pUC ori EGFP Eco0109 I poYpEGFP-C1 HSV TK poly A (3854) BsrG 1 (1323) 4.7 kb SV40 poly A MCS (1330-1417 Kan / Neo f1 SV40 oni ori SV40 Mlu 1 (1642 Dra III (1872) Stul 2577) EGFP 330 TAC AAG TCCGGA CTC AGA TCT CGAG TACC GCG GGC CCG GGA TCC ACC GGA TCTAGA TAA CTG ATC A Bell* BspEI Bglll Xhol Apal Ba Accl Aspl18l sa0al Xbal Sacl Ecn36 II Sac II Restriction Map and Multiple Cloning Site (MCS) of pEGFP-C1. All restriction sites shown are unique. The Xba l and Bc/I sites () are methylated in the DNA provided by BD Biosciences Clontech. If you wish to digest the vector with these enzymes, you will need to transform the vector into a dam host and make fresh DNA.

Explanation / Answer

Rpp has Zero cutter for BamHI, SmaI, SacII, ApaI, HIndIII, KpnI.

We can use any of the above mentioned enzyme to cut Rpp29 out of the plasmid at its 3’ end

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