6. (hemoglobin) How would you make 150 mL of 1.5% agarose (w/v) in 1X running bu

Question

6. (hemoglobin) How would you make 150 mL of 1.5% agarose (w/v) in 1X running buffer? 7. (hemoglobin) How would you make 7.5 L of 70% ethanol? 8. (transformation) How would you make 200 mL LB agar + 100 g/mL Ampicillin? 9. (transformation) If we need 2.5 L of LB +100 ug/mL Ampicillin for all three lab sections, how much total antibiotic do we need to order? Answer in mg. 10. (B-gal) How would you make a 6 fold dilution of X-gal? What would the concentration of the diluted solution be? Ca nNIA ut the DNA is too

Explanation / Answer

6. Ans:

Stock of running buffer solution is 50x. Take 3ml stock sol and add 147 ml mili Q water. To this add 2.25g agorose. Heat the sol in microoven or heat plate. Cool and cast the gel.

7. Ans:

We have 95% ethanol. Dilution calculation by C1V1=C2V2

95*X = 70*7.5

X=5.5263

So take 5.5263L of 95% ethanol and make it to 7.5L by using MQ water/ Distilled water (DW)

8. Ans:

Take 200ml premade LB agar and add 200micro L of ampicilin stock sol. (This is small vol that does not affect con)

9. Ans:

We need 250mg. Because 100micro g per ml. 0.1mg per ml. So0.1*2500=250mg

10.Ans:

20 mg/ml is stock. Take 1ml stck and add 5ml MQ water. Its con will be 3.3333mg per ml.

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