1. Is there any relationship between the Gram-type of an organism and it’s antim
ID: 203402 • Letter: 1
Question
1. Is there any relationship between the Gram-type of an organism and it’s antimicrobial suceptibility? 2. What effect might shaking the cultures have had on the conjugation efficiency? Why? 3. What makes a conjugation experiment more quantitative? 1. Is there any relationship between the Gram-type of an organism and it’s antimicrobial suceptibility? 2. What effect might shaking the cultures have had on the conjugation efficiency? Why? 3. What makes a conjugation experiment more quantitative? 2. What effect might shaking the cultures have had on the conjugation efficiency? Why? 3. What makes a conjugation experiment more quantitative?Explanation / Answer
ANSWER:
1) A Gram positive organism holds onto the primary stain and seems a purple-blue color, while a Gram negative organism holds the second stain and seems pink. A bacterium’s ability to carry onto a stain relies on the structure of their plasma membrane.
2) Gene transfer that spreads antibiotic resistance among microorganism. though conjugation ordinarily happens in surface-associated growth (e.g., biofilms), it's been historically studied in well-mixed liquid cultures lacking spatial structure
Spatial structure suppresses conjugation in surface-associated growth as a result of robust genetic drift ends up inspatial isolation of donor and recipient cells, limiting conjugation to rare boundaries between donor and recipient strains.
3) Bacterial biofilms makes conjugation experiment additional quantitative victimization machine-driven confocal optical device scanning research followed by three-dimensional analysis of cellular biovolumes unconcealed conjugation rates one,000-fold beyond those determined by classical plating techniques.
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