What kind of inhibitor is molecule I if in the presence of both S and I, an ESI
ID: 203467 • Letter: W
Question
What kind of inhibitor is molecule I if in the presence of both S and I, an ESI complex is formed? g) What kind of inhibitor is molecule I if the K of the enzyme for its substrate is not changed, but its Vmax is lowered? h) What kind of inhibitor is molecule if in the Lineweaver Burke plot below, the uninhibited enzyme reaction is represented by the straight line labeled "No inhibitor and the inhibited enzyme reaction is labeled "+I? inhibitor 2. Irreversible inhibitors. Describe how penicillin, acting as an enzyme inhibitor, interferes with the reproduction of bacteria.Explanation / Answer
Answer to first question's subparts
f) .the presented case here clearly shows that an ESI complex is formed . This can happen in non comp. Inhibition or uncompetitive Inhibition . Now , in case of non comp Inhibition , the Inhibitor can bind to enzyme molecules , either in presence or absence of substrate , But Uncompetitive Inhibition requires an already bound substrate to the enzyme ( which ostensibly changes the stereochemi try of unbound enzyme molecule ,and only then the Inhibitor is able to bind to it ) .Since that's the case presented here , it's UNCOMPETITIVE INHIBITION .
g ) - it's a case of typical NON -competitive inhibition , as the addition of Inhibitor Shall lower the maximum rate of catalysis , thereby reducing the Vmax ,but since the Km remains the same ( as same substrate concentration is enough to achieve Vmax 1/2 , hence leaving Km unchanged ) . To understand it better , just give some time to Leave chatelier's principal and Michelis menten constant .the answer would become too lengthy to explain these principles here .
h) - In simplistic terms , the plot in question shows different charechterics for different kinds of inhibition . For comp. Inhibition , the y - intercept changes ,but with same X intercept and different slopes .Vice - versa for X and Y intercepts in case of Non - comp. Inhibition . Uncompetitive Inhibition changes both the X and Y intercepts . Since the Y intercept ( the point where two lines intersect the Y or vertical axis ) , are same , but with different X intercepts , it's a case of competitive inhibition .
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