The three pictuted metabolic schemes are for three different organisms – Pseudom

Question

The three pictuted metabolic schemes are for three different organisms – Pseudomonas, Lactococcus and Mycoplasma. In each diagram, colored lines and nodes represent enzymes that are present in the organism, whereas grey lines and nodes represent enzymes that this organism doesn’t have. You should be able to orient yourself to the diagrams by locating the Acetyl-CoA and Pyruvate nodes on each diagram.

C. What about a mutation in the quinol binding site of the bc1 complex (Complex III) so that it could not accept a quinol?

D. What about a mutation in the gene that encodes glyceraldehyde-3-P dehydrogenase (the enzyme that catalyzes the oxidation of G-3-P)?

Lactococcus:

Mycoplasma:

Pseudomonas:

fatty acids Glycerolipid Carbohydrate Metabolism Amino Acid Metabolism Lipid Metabolism 1 Salfur Fally acid Fatty aci Ene Metabolism lycan etabo gion arginine

Explanation / Answer

Quinol binding site helps in proton conduction pathway, link to electron transfer reaction and it is also provide fundamental atomic structural detail to ubiquinol oxidation. If mutation in any gene which is code for quinol binding site, the quinol can not able to binding that particular site hence whole cycle is disrupted and hinder the ubiquinol oxidation.

Glyceraldehyde 3- phosphate dehydrogenase is the enzyme that catalyze the reaction of glyceraldehydes 3-phosphate to D-glycerate 1,3-biphosphate. It is a enzyme that involve in glycolysis. Here molecules of glucose will be break down to lactate or pyruvic acid and energy. If any mutation that will hinder the function of this enzyme, it will not able to catalyze the reaction and final product and energy will not created.

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