Considering the Metabolic pathways of Pseudomonas aeruginosa: A. Briefly evaluat

Question

Considering the Metabolic pathways of Pseudomonas aeruginosa:

A. Briefly evaluate the metabolism of the organism. How do they make PMF, ATP and reducing power? Do they have a broad or a narrow metabolic capacity?

B. How would deleting the TCA cycle affect this organism?

C. What about a mutation in the quinol binding site of the bc1 complex (Complex III) so that it could not accept a quinol?

D. What about a mutation in the gene that encodes glyceraldehyde-3-P dehydrogenase (the enzyme that catalyzes the oxidation of G-3-P)?

Explanation / Answer

A. The metabolism is common for all the microorganisms. The carbohydrates especially glucose enter into glycolysis and converted into pyruvate molecule and release the ATP by substrate level phosphorylation. They don’t have special organelles like mitochondria to produce ATP. SO the bacterial species uses bacterial membrane to synthesis the ATP molecule.

The pyruvate enters into TCA cycle in cytoplasm itself and produces a NADH and FADH2. These molecules enter into periplasmic membrane or inter membrane space in plasma membrane where the series of electron transport chain complex are present. The NADH and FADH2 reduce and release H+ ions to the inter membrane space. The H+ ions get back to cytosol by ATP synthases and make a proton motive force to generate ATP.

B. Deleting the TCA cycle affect the organism by decreasing the production of major energy carrier like NADH and FADH2 in the cell. So the bacteria unable to produce the energy to meet the demand for doing the normal process in the cell and it will die.

C. Mutation in the quinol binding site of the bc1 complex leads to stop the flow of electron from complex to complex IV. Therefore increase in concentration of electron in the cell leads to death of the cell.

D. Mutation in the gene that encodes glyceraldehyde-3-P dehydrogenase unable to carry the glycolysis further. So again energy demand will take place in the cell.

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