2. Protease specificity (10 points). The following data were collected for pepti

Question

2. Protease specificity (10 points). The following data were collected for peptide bond cleavage of various substrates by a protease. The location of the peptide bond cleaved is marked with an asterisk. KM (mM 4 -1 Substrate EMTA*G EMTA*A EMTA*F EMTI*F 24 30 0.5 9 18 a. If this enzyme was exposed to a mixture of these peptides (each peptide at the same concentration), which peptide would be digested most rapidly? Briefly explain your reasoning. (1-2 sentences b. On the basis of these data, suggest which features of the substrate dictate the specificity of the enzyme. Briefly explain your reasoning. (1-2 sentences)

Explanation / Answer

answer a) the enzyme whose Kcat is high and low km will efficiently work in the peptide solution and would able to digest it easily so the answer of this question from above table is EMTA*A will able to digets the peptide rapidly.

answer b) specificity of enzyme refers to the tendency of enzyme to catalyse the specific set of reaction. so the answer will be kcat/km is the representation of the specificity of enzyme.

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