Lee Hartwell isolated a mutant allele of CDC28 that resulted in the inability of

Question

Lee Hartwell isolated a mutant allele of CDC28 that resulted in the inability of haploid yeast cells, which have 16 chromosome and 12 X10^6 base pairs, tom arrest prior to entry into S-phase of the cell cycle. Using FACS analysis, what would you expect the fluorescent profile to look like on the isolated mutant cells?

A) Even peaks of 2C and 4C DNA content

B) One peak of 2C DNA content

C) A broad peak from 2C to 4C content

D) A single peak at 1C DNA content

E) No fluorescent signal because the cells could not replicate their DNA

Explanation / Answer

The fisson cell cycle of yeast can be measured by technique called Flow cytometry and Fleuorescenc activating cell sorting technique.At normal condition the cytokinesis take place by the end of S phase.The cells in G1 and S phase is binuclear.They have one complete genome 1DNA at stage G1 and 2DNA at G2 stage.The cells got arrested in G1 phase(cell arrested prior to S pgase) represent single cell with single nucleus termed as 1DNA with lower FSC (forward light scateering)and SSCside scaterring (values).

Option C-A single peak with 1C DNA Content.

Related Questions

Navigate

• Browse (All)
• Browse L
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.