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of the OSU operon which contains genes bev1 and bev2. One reg mutant has a defec

ID: 204291 • Letter: O

Question

of the OSU operon which contains genes bev1 and bev2. One reg mutant has a defect in the DNA binding site, the other has a defect in the genes encoding a protein that binds to that site (loss of function). A. Is the DNA-binding protein a positive or negative regulator of gene expression? How do you know? To determine which mutant has a defect in the site and which one has a mutation in the binding protein, you decide to do a merodiploid analysis. Assuming that you can assay levels of Bev1 and Bev2 proteins, what results do you predict for for Bevi and Bec2 expression given the two strains below i and ii) if reg2 encodes the regulatory protein and reg1 is the regulatory site? B. i. Freg1- reg2+ bev1- bev2+/ reg1+ reg2+ bev1+ bev2- i. F'regl+ reg2- bev1-bev2+/ reg1+ reg2+ bev1+ bev2-

Explanation / Answer

A) Constitutive expression is seen in mutants, this is due to the mutation either in the repressor protein that unables to bind to operator or in the operator, the DNA binding site. The DNA binding protein is a negative regulator of gene expression, Repressor.

B) reg1 is the DNA binding site, operator and reg2 is the negative regulator of gene expression.

i) In this merodiploid, the concentration of synthesized Bev2 will be high as compared to the concentration of Bev1. Because Bev2 will be constitutively expressed due to the mutation in reg1. Bev2 - very high concentratiom and Bev1 - high concentration

ii) Again in this merodiploid, Bev2 will be synthesized constitutively due to the mutation in repressor, that unables it to bind to operator. Bev2 - very high concentratiom and Bev1 - high concentration

C) reg1 is the negative regulator of gene expression and reg2 is the DNA binding site, operator

i) reg1 or repressor is mutated that unables it to bind to operator causing it to express constitutively. Therefore, bev2 will be synthesized constitutively. bev2 - very high concentration and bev1 - high concentration

ii) reg2 or operator is mutated, unabling repressor to bind to it, causing bev2 to express constitutively. bev2 - very high concentration and bev1 - high concentration

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