Need help with question 6 and 7 6. Cytochalasin B strongly inhibits certain form
ID: 204294 • Letter: N
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Need help with question 6 and 7 6. Cytochalasin B strongly inhibits certain forms of cell motility. In classic experiments that defined its mechanism, short lengths of actin microfilarmeisend were decorated with myosin heads and then mixed with actin subunits the critical concentration in the presence(+) or absence () of cy Assembly of actin microfilaments was assayed by examining samples electron microscopy. The figure to the right shows results desc appearance of actin microfilaments formed in the presence and cytochalasin B. The decorated actin microfilaments (gray circles present before the addition of actin monomers are shown at the top of each set of three. Microfilament = ere, wwwww above tochalasin B by describing the absence of lines) s present after increasing times of incubation with actin monomers (red circles) are shown in the next two rows. A. (3pts) Please propose a molecular mechanism for how cytochalasin B affects the dynamics of actin microfilaments that is consistent with the EM data B. (2pts) Why was it informative to decorate the actin microfilaments with myosin heads before adding mixing with actin subunits? 7. (4pts) Many transport vesicles carry both kinesins and dyneins. Please provide a reason why this might be the case.Explanation / Answer
A. As Cytochalasin B directly inhibits the function of cytokinesis the process of cell division as a result two daughter nuclei are formed that directly affects actin filament polymerization functions. Due to this role of cytochalasin B and it's less toxicity it has great potential to be used as a drug molecule specially as a anti-cancer drugs.
B. In the presence of cytoclalasin B the focal contractions of broken actin filaments are enhanced in the presence of myosin. As it directly affect the contraction of actin filaments the process of cell motility is ceased under it's presence.
7. Kinesins and dyneins are type of motor protein, Kinesins helps in moving towards positive ends of tubules and Dyneins towards the negative end of the microtubules.
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