Exam Instructor: J. Amitto Iu: A Matching For the following lems, motch each sel

Question

Exam Instructor: J. Amitto Iu: A Matching For the following lems, motch each selentific ternwith the descriplian fej that hest fit u. When complometary DNA strands come topeilier bHost DN struetures that sccent and incorporale DNA resulting in ONA c. Nshon. agged" piece ofDNA complemeriary to le DNN of intares d. The liquid nbove a peller afler cenkrifagation e. The saled-up process by whidh cells sre grown undey oplimal conditioes fer masíimun ce product formation F FDA regulated nanufacturing pronccol E The desired result of endomsvclease aciviay for the purpose of DN producton dik h Cells treated with calcium chi i Probes used specifically 1o initiate the poltymerase ehain reacion j Whcn cells grow rapidly such that in cech cell eseve, the cell sount da Probe 2 Priiners 3, Hybridlize Competent cells 5 Fementation 6. Exponential growth , cGMP 8, Vector 9, Supernatant 10 Sticky ends For the following items, match rach technique with its correspandinyg hast DNA argunismu a bacteria b. manmalian cells e, viruses 11. transduction 12. transfection 13, transformation For the following iems, match each ensyme with its corresponding actvity (a-e). a, cuts DNA b, pastes pieces of DNA together c bursts cells open 4. lysozyme 5. restriction enzyme 16. DNA ligase

Explanation / Answer

1.- c

Probe are short tagged piece of DNA complimentary to DNA of interest.

2.- i

Probes used in PCR to initiate polymerisation is called primer.

3.- a

Hybridization is process of binding of complimentary sequences.

4.- h

Competent cells have increased ability to uptake DNA.

5.- e

Fermentation is scaled up culturing of cell under optimal conditions.

6.- j

When cells doubles in each cycle then it is called exponential growth.

7.- f

cGMP is FDA manufactured protocol.

8.- b

Vector are DNA that accept DNA in rDNA practices.

9.- d

Liquid above pallet in centrifigation is called supernatant.

10.- g

Sticky end are desired result of endonucleases.

11.- c

Transduction is mechanism of DNA transfer in viruses.

12.- b

Transfection is process of transferring piece of DNA in animals.

13. - a

DNA transfer in bacteria is by Transformation.

14.- c

Lysozyme brust open the cells.

15.- a

Endonucleases cuts DNA.

16.- b

Ligases paste the DNA.

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