111 T-Mobile 2:55 PM * 75% bbhosted.cuny.edu Study Guide Chapter 1 BI0472 Februa
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111 T-Mobile 2:55 PM * 75% bbhosted.cuny.edu Study Guide Chapter 1 BI0472 February 22 2018 1. What is major the function of restriction enzymes in recombinant DNA technology? 2. Summarize the differences between Classes 1 and m restriction enzymes? 3. What is a Blunt end digestion by restriction enzyme? 4. Define the role of a promoter in the process of transcription? 5. How will you isolate mRNA if you need to prepare a cDNA library? 6. What is the major difference between genomic DNA and cDNA? 7. What is a Ti plasmid-where it is found and what is its role in engineering GMO-Plants? 8. Why we cannot express a glycoprotein in bacteria? 9. What is the advantage of expressing a protein in Yeast rather than Bacteria? 10. What are Cosmid vectors? 11. What do you mean by the term epigenetics? 12. What do you understand by the term gene activation and gene silencing?Explanation / Answer
1.
Restriction enzymes are endonucleases used to cut DNA at specific sites and recombine them as when and where needed. They are of critical importance in recombinant DNA technology.
2.
Type1 class: these restriction enzymes cleaves DNA randomly away from the recognized sequences.
Type2 class: : these restriction enzymes cleaves DNA little away from the recognized sequences.
Type3 class: : these restriction enzymes cleaves DNA within the recognized sequences.
3.
WWhen restriction enzymes cut DNA at opposite base, then blunt ends are formed which are used to join only with blund end fragments.
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