Q36. You have a concentrated stock solution of BSA at 200 g/mL, but need a 50 g/
ID: 206424 • Letter: Q
Question
Q36. You have a concentrated stock solution of BSA at 200 g/mL, but need a 50 g/mL solution of BSA. What volume of the original stock solution is needed to make 500 L of 50 g/mL? Q37. The protocol you are following specifies 1 mL of E. coli broth culture to be included within a total reaction volume of 10 mL to give final concentration of 1 x 105 cells/mL in the reaction. What is the required concentration of your original E. coli culture? Q38. You have a stock solution of MgCl2 of 20 mM concentration. If you use 1 of this concentrated MgCl2 in your PCR for a final reaction volume of 20 L, what will be the concentration of MgCl2 in your PCR?Explanation / Answer
Q.36 This can be solved by C1xV1= C2x V2
200x X = 500x 50
X= 125microlitre
So 125 microlitre will be needed to make 500 microlitre solution.
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