a.) 42 degree Celsius b.) 37 degree Celsius c.) 100 degree Celsius d.) 0 degree
ID: 20681 • Letter: A
Question
a.) 42 degree Celsius
b.) 37 degree Celsius
c.) 100 degree Celsius
d.) 0 degree Celsius
e.) 121 degree Celsius
f.) -80 degree Celsius
g.) room temperature
Using the above choices answer the following questions.
1.) temperature for keeping competent cells alive.
2.) temperature for denaturing protein in preparation for SDS-PAGE
3.) temperature for an autoclave when sterilizing
4.) temperature for "shocking" competent cells so that they take up plasmid DNA
5.) temperature for allowing cells to express beta-lactamase after taking up DNA
6.) temperature at which boiled agarose solidifies into a gel
7.) temperature at which competent cells are stored
Explanation / Answer
Using the above choices answer the following questions.
1.) temperature for keeping competent cells alive.----------b.) 37 degree Celsius
2.) temperature for denaturing protein in preparation for SDS-PAGE---------c.) 100 degree Celsius
3.) temperature for an autoclave when sterilizing----------e.) 121 degree Celsius
4.) temperature for "shocking" competent cells so that they take up plasmid DNA----------0 degree Celsius
5.) temperature for allowing cells to express beta-lactamase after taking up DNA-42 degree Celsius
6.) temperature at which boiled agarose solidifies into a gel g.) room temperature
7.) temperature at which competent cells are storedf.) -80 degree Celsius
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