3.In a DNA library, each plasmid can contain more than one gene. This is not tru

Question

3.In a DNA library, each plasmid can contain more than one gene. This is not true for a cDNA library because?

a. one molecule of cDNA contains one gene

b. different restriction enzymes are used

c. host cells cannot be transformed when there is more than one gene in the plasmid

d. the vectors used in cDNA are different

4.You wish to create a transgenic mouse that is completely hairless. You introduce the appropriate mutation into embryonic stem cells and implant those cells into early embryos. Those embryos are placed in a surrogate mouse who then gives birth to the first generation of mice. These mice are further bred.

which of the following is (are) true?

a. first generation is completely hairless

b. second generation is completely hairless

c. third generation is completely hairless

d. second geneeration is heterozygous

e. second genertion are crossed with each other

f. first generation are crossed with each other

Explanation / Answer

Question 3

cDNA or complementary DNA is prepared from mRNA obtained from cells. Since one complete mRNA molecule will be one gene hence one cDNA molecule synthesized by using one mRNA as a template (by reverse transcriptase) will also be one gene hence the answer is (a)

Question 4

Only one copy of the gene (the mutated one) shall give hairless condition since the first generation is heterozygous it won't show the phenotype but when selfed few second generation individuals will show the desired phenotype. Hence the only correct answer is (f) the first generation are crossed with each other.

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