Can anyone please Help me with the following question. It has more than one part

Question

Can anyone please Help me with the following question. It has more than one part. The questions are based on the 2 articles below. Below are the links to the article:
http://www.microbiologyresearch.org/docserver/fulltext/ijsem/65/5/1426_ijs000113.pdf?expires=1520888319&id=id&accname=guest&checksum=FB08379551C46FC10AC5A24438E633F9


http://www.rcn.montana.edu/Publications/Pdf/2007/bryant%20et%20al%202007.pdf

1a)Where and how was this organism isolated?

1b) What are metagenomics techniques and how were they applied to the discovery of this organism?
1c) Describe the significance of Figure 1 in the Science paper.

Can anyone please Help me with the following question. It has more than one part. The questions are based on the 2 articles below. Below are the links to the article:
http://www.microbiologyresearch.org/docserver/fulltext/ijsem/65/5/1426_ijs000113.pdf?expires=1520888319&id=id&accname=guest&checksum=FB08379551C46FC10AC5A24438E633F9


http://www.rcn.montana.edu/Publications/Pdf/2007/bryant%20et%20al%202007.pdf

1a)Where and how was this organism isolated?

1b) What are metagenomics techniques and how were they applied to the discovery of this organism?
1c) Describe the significance of Figure 1 in the Science paper.


http://www.microbiologyresearch.org/docserver/fulltext/ijsem/65/5/1426_ijs000113.pdf?expires=1520888319&id=id&accname=guest&checksum=FB08379551C46FC10AC5A24438E633F9


http://www.rcn.montana.edu/Publications/Pdf/2007/bryant%20et%20al%202007.pdf

1a)Where and how was this organism isolated?

1b) What are metagenomics techniques and how were they applied to the discovery of this organism?
1c) Describe the significance of Figure 1 in the Science paper.

Explanation / Answer

1. Novel anoxygenic photoheterotrophic which is a member of the phylum Acidobacteria , Chloracidobacterium thermophilum strain B sp. nov., was isolated from a cyanobacterial enrichment culture derived from microbial mats associated with Octopus Spring,

2.

Metagenomic project workflow

First the prokaryotic cell is extracted from environmental samples (e.g. soil, sediment, seawater). Subsequently, the total DNA content (the metagenome) is being extracted and than purified. During the procedure purified DNA is sheared and contains fragments of different size. DNA fragments (inserts) of the appropriate size are then cloned into a cloning vector and transformed into a host cell. Here, the type of cloning vector is of crucial importance. The result is thousands of cells, each carrying a DNA fragment from the metagenome. All cells together build up the metagenomic library. Cloning can also be skipped, for example if 454-sequencing is used (see below). Metagenomics can be used for different purposes and therefore the project steps vary accordingly.

C. not clear

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