Name: Section Lab Homework 7 Due at the statt of lab The data below represent ce

Question

Name: Section Lab Homework 7 Due at the statt of lab The data below represent cell counts and optical density readings of a bacterial culture of taken over a period of one hour. Time (min) | Viable Count (CFU/ml)Don 15 30 45 60 7.8 x 10 1.3 x 10 4.3 x 10 6.4 x 10 1.0 x 10 0.014 0.020 0.063 0.087 0.199 1. Plot the data on one piece of semi-log graph paper by hand. Label your axes. (5 points) 2. Draw trend lines on your graph Use your graph to calculate the doubling time. Show yoru work on your graph. Put your doubling time here: (3 points) 3. Use your graph to calculate the viable cell count at an OD reading of 0.075. Put your answer here. Show your work on your graph. (1 point) 4. In the remaining space, draw dilution scheme that would put 30-300 colonies on a plate based on your measurements. Look to the lab book for models as to how to draw the dilution scheme. Your scheme should include dilution tubes, plates and volumes. It must be hand drawn in the space provided. (3 points)

Explanation / Answer

The arrow is representing the doubling time because here cells are doubled from 7.3 x 105 to 13 x105 .

The trend line is showing beside the blue line of growth.

the X axis has Time in minutes.

Time (min) Viable count (x10^5) 0 7.8 15 13 30 43 45 64 60 100

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