pO,(kPa) 10. The following shows an Fas fragment of an antibody, with the variab
ID: 210279 • Letter: P
Question
pO,(kPa) 10. The following shows an Fas fragment of an antibody, with the variable region on the right. Please explain how the 3-dimensional structure of this protein facilitates the ability of antibodies to develop specificity against a large number of different antigens (2 pts). Fob fragments of antibodies raised against a specific protein of interest are sometimes used in X-ray crystallography to help that protein crystallize. Suggest why this might work (1 pt). Note that for a crystal to form, you must have regular protein-protein contacts throughout the crystal. Also, what could be another workaround to solve the 3-dimensional structure of a protein that cannot be crystallized (1 pt)? BONUS QUESTIONS-pick one of the following two. If you show work on both question, please Indicate which one you want us grade. B1. You have a solution of 50 mL of a 15 mM amino acid that displays a net negative charge at pH 7. After the addition of 27 m 10 mM HCI, the pH has changed to 4.5. What is the identity of the amino acid (10 pts.)? Explain your answer and show your w 82. Draw the full and detailed mechanism by which the GroEL/GroES system in bacteria can help proteins fold into their con native structures, including the schematic representation of the protein complex and all co-factors that are used during thi (10 pts).Explanation / Answer
The Fab part of antibody is responsible for providing specificity to antigen-antibody interactions. This region contains a large number of complementarity determining regions (CDRs). These CDRs are different for different antigens; so, a specific CDR region will bind to a specific antigen only. So, every antibody is specific for a specific antigen.
The Fab fragment has two regions, one is the constant region and the other is the variable region. The variable region contains a CDR region (discussed above). This region helps maintain specificity in the antigen-antibody interaction.
Now, when CDR region (of Fab) of a specific antibody binds to the antigen, then an agglutination reaction occurs. The antigen-antibody complex is found in the solution in the form of clumps or you can say crystals.
The figure shown sideways (in the question) shows a clear 3D structure of an antibody. There is a looped structure present in the 3D structure of antibody. The antigen goes and fits into this central loop.
**Please post the other question separately.
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