This is from a study guide that I\'ve already answered, but wanted to compare my

Question

This is from a study guide that I've already answered, but wanted to compare my answers in case I missed anything or misunderstood the question. Thank you!!!

What does the term transgenic mean?

What are some of the concerns around genetically modified organisms?

What distinctive features of the CRISPR loci attracted attention to these regions in Prokaryotes? (Hint: what does CRISPR stand for?)

Diagram the defensive complex that is formed from the CRISPR locus to attack foreign DNA invading bacteria. What are the components? What are features necessary in the targeted foreign DNA? Describe the interaction between the targeting complex and the foreign DNA. What happens when they interact? Include the following terms: tracrRNA, crRNA, CAS-9, PAM site

In using the CRISPR-CAS 9 system to modify DNA describe the two possible pathways after the double strand break occurs Non-Homologous End Joining (NHEJ) and Homologous Direct Repair (HDR) and the possible outcomes of each.

Explain the natural CRIPSR components are modified to use in genetic engineering? Assume you can introduce a plasmid with the CAS-9 gene, a small guide RNA (sgRNA) targeting a particular gene, and a homologous gene with a modified sequence.

What variations on how CRISPR is used to manipulate cells?   

What makes the CRISPR-CAS 9 system a game-changer in terms of genetic engineering?

Explanation / Answer

1.Transgenic : It means that the genome of an organism is altered by introducing a fragment of genome from another genus or species. It has various applications like vaccine production, monoclonal antibody prduction.

2. The concerns related to genetically modified organisms are that they may pose health and environmental risk. If the GMO is a food crop, people are skeptical of the safety of edibility.

3. CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats. Bacterial genome contains many regularly spaced short palindromic sequences in their genome which plays an important role in immunity.

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