Which FACS plot represents a population of S. pombe that have been transferred i
ID: 210415 • Letter: W
Question
Which FACS plot represents a population of S. pombe that have been transferred into a minimal liquid media ( i.e. media containing no growth factors, with barely enough essential amino acids and glucose to permit cell survival) then incubated for 6 hours prior to analysis?
(Note, assume that FACS plot #1 shows analysis of a population of S. pombe replicating asynchronously (i.e. each cell within a population is at a random stage of the cell cycle) in culture. In the rich media assume the yeast have a cell cycle time of 100 min, with G1=50 min; S = 25 min; G2 = 15 min and M = 10 min).
#1 #2 #3 #4 #5 #6 cells in G phase cells in Gaand M phases cells in S phase 2 relative ameunt of DNA per cell (arbitrary units) #2 #3 #4 #5Explanation / Answer
#3 is the answer, in case of absence of nutrients cell division will not proceed to fullest. Hence we can say that 2 representing G2 and M phase where the division takes place is absent in #3 hence we can conclude that it the assay where the division has not taken and bare survival is happening.
Related Questions
drjack9650@gmail.com
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.