1. For the following Prokarvotic DNA strand, show the mRNA which would be transc
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Question
1. For the following Prokarvotic DNA strand, show the mRNA which would be transcribed from this strand and show the polypeptide which would be produced if the mRNA were translated. (See a codon chart for reference). 3' 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 GCATTACG CTT TA C T CTACATT G G 5' DNA strand mRNA: Protein Polypeptide 2. In the DNA strand shown in the previous problem, what would be the effect on the resulting polypeptide if the following occurred? Be specific ie write out the result: Codon and amino acid. A. Delete base 17? B. Changing A to G at base 13? C. Changing C to A at base 14? D. Changing C to T at base 9? 3. What are the names of these types of mutations? Mutation A Mutation B Mutation C Mutation D On the DNA strand above, circle the areas that would be affected by UV radiation. Why doesn't UV light kill all of the bacterial cells?Explanation / Answer
Answer 1.
DNA Strand: 3' - GCATTACGCTTTACTCTACATTGG - 5'
cDNA Strand: 5' - CGTAATGCGAAATGAGATGTAACC - 3'
mRNA: CGUAAUGCGAAAUGAGAUGUAACC (From Start Codon AUG to Stop Codon UAA)
Protein Polypeptide: MRNEM (As per protein single alphabet codes)
Answer 2.
A.
DNA Strand: 3' - GCATTACGCTTTACTCACATTGG - 5'
cDNA Strand: 5' - CGTAATGCGAAATGAGTGTAACC - 3'
mRNA: CGUAAUGCGAAAUGAGUGUAACC (From Start Codon AUG to the end as Stop codon is mutated)
Protein Polypeptide: MRNECN (As per protein single alphabet codes)
B.
DNA Strand: 3' - GCATTACGCTTTACTCTGCATTGG - 5'
cDNA Strand: 5' - CGTAATGCGAAATGAGACGTAACC - 3'
mRNA: CGUAAUGCGAAAUGAGACGUAACC (From Start Codon AUG to Stop Codon UAA)
Protein Polypeptide: MRNET (As per protein single alphabet codes)
C.
DNA Strand: 3' - GCATTACGCTTTAATCTACATTGG - 5'
cDNA Strand: 5' - CGTAATGCGAAATTAGATGTAACC - 3'
mRNA: CGUAAUGCGAAAUUAGAUGUAACC (From Start Codon AUG to Stop Codon UAG)
Protein Polypeptide: MRN (As per protein single alphabet codes)
D.
DNA Strand: 3' - GCATTACGTTTTACTCTACATTGG - 5'
cDNA Strand: 5' - CGTAATGCAAAATGAGATGTAACC - 3'
mRNA: CGUAAUGCAAAAUGAGAUGUAACC (From Start Codon AUG to Stop Codon UAA)
Protein Polypeptide: MQNEM (As per protein single alphabet codes)
Answer 3.
Mutation A: Deletion, Frame-shift
Mutation B: Substitution, Transition, Mis-sense mutation
Mutation C: Substitution, Transversion, Nonsense mutation
Mutation D: Substitution, Transition, Mis-sense mutation
Answer 4.
DNA Strand: 3' - GCATTACGCTTTACTCTACATTGG - 5'
UV radiation will cause two nearby Thymine's to form a bond between themselves leading to bulky locations affecting the DNA double-helical structure, this is called thymine dimer
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