8. Which of the following media can be used to determine Hydrogen sulfide produc
ID: 210926 • Letter: 8
Question
8. Which of the following media can be used to determine Hydrogen sulfide production? a SIM media b. KIgler's Iron agar slant cRussel Double Sugar (RDS) slant d. A and B can both be used satisfactonily e. Aand C can both be used satisfactorily 9 Indole is a by product of the metabolic breakdown of the amino acid Tryplophsn used by some orgenisms. The presence of Indole can be readily demsonstrated by using wbich of the Sollowing a Harritt's reagent b. Oxidase reagen c. Ferric Chloride selution d Kovac's r Gram's lodine What test reagent is needed to demonstrate the resalts of Voges Prosksest O Barrin's reagents A and b. Methyl red Nitrate reagents A and e Ferric chlonide solution 11. Fersentation may be definod as the incomplete oxidation of a. Stanch in the presence of oxyeen h Starch in the absence of osygen c. Glucose or other monosaccharides in the presence of oxyees d Glucose or other monosaccharides in the absence of oxyges eUrca in the absence of oxypen 12. A Staphyloccccal isolate found to be positive for mannitol Senmcntation is likely to be d Either A or C e Either A or B If optochin sensitivity is indicated in a blood agar plate inoculaseod with Sptoccun, the ba a Sreptococcus agalactiae d. Streptococcus pumoniae Shigella and Salmonella are a. b. c. d. Both positive for lactose fermentation Both positive for hydrogen sulfide production Both negative for urease activity Both negative for indole production annitol Salt Agar contains mannitol, 7.5% sodium chloride, and phenol red. The rd dium is a. To select for all gram positive bacteria b. To select for all gram negative bacteria To inhibit organisms other than Staphylococci None of the above is applicableExplanation / Answer
8) The correct option is d) A and B can both be used satisfactorily.
The SIM and Klinger Iron slant can be used satifactorily as a medium for the determination of H2S.
9) The correct option is d) Kovac's reagent.
Kovac's reagent can be used to determine the presence of Indole.
10) The correct option is a) Barritt's Reagent A and B.
Barritt's Reagent A and B is used in Voges-Proskauer test for detection of acetoin production by bacterial culture.
11) The correct option is d) Glucose or other monosaccharides in the absence of oxygen.
Fermentation occurs due to the incomplete oxidation of glucose or any other monosaccharides in the absence of oxygen.
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