all T-Mobile c @ 46% D. 5:46 PM Baltimore March 7 12:59 PM 4G Edit LIVE 27. A le

Question

all T-Mobile c @ 46% D. 5:46 PM Baltimore March 7 12:59 PM 4G Edit LIVE 27. A lechnique that could be used to demonstrate that Gutt and Gluts cancer cells as comparod to normal cells is A) DNA seguencing B) Mass spectrometry C) Wessem bloting D) Thin layer chromatography E) Electron microscopy 28, Allosteric inhibitors of enzyme activity A) Likely to compete with the substrate for the active site B) Can be competed out by excess substrate C) Bind to site distinct from the active site Cannot be competed out by excess substrate Both choices C and D 29. During the pulse chase experiment in pancreas cells the labeled peroteins start off isvon the A) Nucleus, nucleoid B) Mitochondria, chloroplast and end up in/on the bosomes, vesicles D) Vesicles, nucleus E) All of the above 30. If one homogenizes liver cells, separates the membranes from the cytoplasm, and then adds epinephrine to only the cytoplasmic portion, CAMP will be produced. 8) G-proteins will be activated. C) adenylyl cyclase will be activated D) calcium ions will be released. E) none of the above

Explanation / Answer

27 C western blottng: overexpresson of a protein can be checked with this technique

28. E Both choices C and D: Allosteric inhibitors bind to a site other than active site... therfore it does not compete with the substrate

29. C Ribosomes and vesicles.. proteins are synthesized in ribosomes and then carried to plasma membrane via secretory vesicles.

30. E none of above... Epinephnephrine is a hormone that is water soluble and cannot cross plasma membrane. It binds to beta adrenegic receptors to produce cAMP through a cascade..if only cytoplasmic portion is used then nothing will be produced.

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