3. From an immortalized human HeLa cell line with wild-type p53 genes, you have

Question

3. From an immortalized human HeLa cell line with wild-type p53 genes, you have derived a new cell line that lacks both copies of the gene. You treat the original and newly derived cell lines with the anticancer drug doxorubicin, which can activate the p53 pathway in the cell by stalling DNA replication forks and inducing double-strand breaks in DNA. You measure cell proliferation in the presence of different doses of the drug in each of the two cell lines, and plot the results as shown in the graph below. Which cell line (A or B) do you expect to be the original HeLa lime? point) 0.1 10 100 Concentration of doxorubicin (nM, log scale)

Explanation / Answer

Which cell line (A or B) do you expect to be the original HeLa line?

B) B

Cell line A does not have the wild-type p53 gene, So the anticancer drug will not be able to activate p53 pathway. Cell line B shows the effect of anticancer drug in this graph.

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